Product Name :
WNK1 Recombinant Rabbit Monoclonal Antibody [JG36-91]
Predicted band size :
225/250/280/300 kDa kDa
Observed band size :
250/300 kDa
Synonyms:
Erythrocyte 65 kDa protein antibody HSAN2 antibody HSN2 antibody hWNK1 antibody KDP antibody KIAA0344 antibody Kinase deficient protein antibody MGC163339 antibody MGC163341 antibody p65 antibody 展开
Function :
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Synthetic peptide within Human WNK1 aa 33-82 / 2382.57595-23-0 site
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IHC-P
Coupling :
unconjugated
Clone number:
JG36-91
RRID:
AB_3070839
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.Price of 141215-32-9 Aliquot store at -20℃.PMID:33486519 Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
225/250/280/300 kDa
Positive control :
A549 cell lysates, human skin tissue, human breast cancer tissue, mouse testis tissue, rat kidney tissue.